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multisite gateway lr reaction  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multisite gateway lr reaction
    Multisite Gateway Lr Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multisite+gateway+lr+reaction/multisite+gateway+lr+reaction/pm40083573-29-28-32
    Average 90 stars, based on 1 article reviews
    multisite gateway lr reaction - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: TALE-based C-to-T base editor for multiple homologous genes with flexible precision.
    Article Snippet: .. After assembly-step 2, the entry vectors harboring the whole coding sequences of left or right TALECD, the entry vector 2 and the destination vector were assembled by a multisite Gateway LR reaction (ThermoFisher Scientific, Waltham, MA, USA). ..

    Article Title: The Iron Deficiency-Regulated Small Protein Effector FEP3/IRON MAN1 Modulates Interaction of BRUTUS-LIKE1 With bHLH Subgroup IVc and POPEYE Transcription Factors.
    Article Snippet: .. In a multisite Gateway LR reaction (Thermo Fisher Scientific, Darmstadt, Germany), both genes were transferred simultaneously into destination vector pBiFCt-2in1-NN (N-terminal nYFP and cYFP fusions) (Grefen and Blatt, 2012), to create pBiFCt-2in1-NN: FEP3:BTSL1, pBiFCt-2in1-NN:PYE-BTSL1, pBiFCt-2in1-NN:PYE-BTSL1-C and pBiFCt-2in1-NN:ILR3-BTSL1-C. ..

    Article Title: The Iron Deficiency-Regulated Small Protein Effector FEP3/IRON MAN1 Modulates Interaction of BRUTUS-LIKE1 With bHLH Subgroup IVc and POPEYE Transcription Factors
    Article Snippet: .. In a multisite Gateway LR reaction (Thermo Fisher Scientific, Darmstadt, Germany), both genes were transferred simultaneously into destination vector pBiFCt-2in1-NN (N-terminal nYFP and cYFP fusions) , to create pBiFCt-2in1-NN: FEP3:BTSL1, pBiFCt-2in1-NN:PYE-BTSL1, pBiFCt-2in1-NN:PYE-BTSL1-C and pBiFCt-2in1-NN:ILR3-BTSL1-C. ..

    Sequencing:

    Article Title: Construction of tomato plants with suppressed endo-β-N-acetylglucosaminidase activity using CRISPR-Cas9 mediated genome editing.
    Article Snippet: High mannose-type free N-glycans with a single N-acetyl-D-glucosamine (GlcNAc) residue at the reducing end (GN1-HMT-FNGs) are produced by cytosolic endo-β-N-acetylglucosaminidase (EC:3.2.1.96) (ENGase) and are ubiquitous in differentiating and growing plant cells.. To elucidate the physiological functions of HMT-FNGs in plants, we identified the ENGase gene in tomato (Solyc06g050930) and detected ENGase activity and increased production of GN1-HMT-FNGs during tomato fruit maturation.. However, the precise role of GN1-HMT-FNGs in fruit maturation remains unclear.

    Clone Assay:

    Article Title: Construction of tomato plants with suppressed endo-β-N-acetylglucosaminidase activity using CRISPR-Cas9 mediated genome editing.
    Article Snippet: High mannose-type free N-glycans with a single N-acetyl-D-glucosamine (GlcNAc) residue at the reducing end (GN1-HMT-FNGs) are produced by cytosolic endo-β-N-acetylglucosaminidase (EC:3.2.1.96) (ENGase) and are ubiquitous in differentiating and growing plant cells.. To elucidate the physiological functions of HMT-FNGs in plants, we identified the ENGase gene in tomato (Solyc06g050930) and detected ENGase activity and increased production of GN1-HMT-FNGs during tomato fruit maturation.. However, the precise role of GN1-HMT-FNGs in fruit maturation remains unclear.

    Expressing:

    Article Title: The mitochondrial orf117Sha gene desynchronizes pollen development and causes pollen abortion in Arabidopsis Sha cytoplasmic male sterility.
    Article Snippet: Ac ce pte d M an us cri pt © The Author(s) 2024.. Published by Oxford University Press on behalf of the Society for Experimental Biology.. All rights reserved.

    TALENs:

    Article Title: The mitochondrial orf117Sha gene desynchronizes pollen development and causes pollen abortion in Arabidopsis Sha cytoplasmic male sterility.
    Article Snippet: Ac ce pte d M an us cri pt © The Author(s) 2024.. Published by Oxford University Press on behalf of the Society for Experimental Biology.. All rights reserved.

    Control:

    Article Title: The mitochondrial orf117Sha gene desynchronizes pollen development and causes pollen abortion in Arabidopsis Sha cytoplasmic male sterility.
    Article Snippet: Ac ce pte d M an us cri pt © The Author(s) 2024.. Published by Oxford University Press on behalf of the Society for Experimental Biology.. All rights reserved.

    Article Title: The brassinosteroid receptor gene BRI1 safeguards cell-autonomous brassinosteroid signaling across tissues
    Article Snippet: For transgene constructs, attB-flanked coding sequences were synthetized ( BRI1 REC , BRI1 KDD ) or amplified ( BIR3 EXT -BRI1 INT , BRI1, BRL1, BRL2 and BRL3 ) by PCR and recombined into the pDONR221 vector (Invitrogen) to produce the pEN-L1-CDS-L2 clones. .. Protein fusions under the control of different promoters were generated by recombining pEN-L4-promoter-R1, pEN-L1-CDS-L2 and pEN-R2-CITRINE-L3 plasmids into a destination vector by Multisite Gateway LR reaction (Thermo Fisher Scientific). ..

    Article Title: The brassinosteroid receptor gene BRI1 safeguards cell-autonomous brassinosteroid signaling across tissues
    Article Snippet: The BRI1 REC sequence was generated by the GeneOptimizer recoding algorithm (Thermo Fisher Scientific) using the normal BRI1 sequence as a template (see the Supplementary Materials, data S2 for sequences). .. Protein fusions under the control of different promoters were generated by recombining pEN-L4-promoter-R1, pEN-L1-CDS-L2, and pEN-R2-CITRINE-L3 plasmids into a destination vector by Multisite Gateway LR reaction (Thermo Fisher Scientific). ..

    Generated:

    Article Title: The brassinosteroid receptor gene BRI1 safeguards cell-autonomous brassinosteroid signaling across tissues
    Article Snippet: For transgene constructs, attB-flanked coding sequences were synthetized ( BRI1 REC , BRI1 KDD ) or amplified ( BIR3 EXT -BRI1 INT , BRI1, BRL1, BRL2 and BRL3 ) by PCR and recombined into the pDONR221 vector (Invitrogen) to produce the pEN-L1-CDS-L2 clones. .. Protein fusions under the control of different promoters were generated by recombining pEN-L4-promoter-R1, pEN-L1-CDS-L2 and pEN-R2-CITRINE-L3 plasmids into a destination vector by Multisite Gateway LR reaction (Thermo Fisher Scientific). ..

    Article Title: The brassinosteroid receptor gene BRI1 safeguards cell-autonomous brassinosteroid signaling across tissues
    Article Snippet: The BRI1 REC sequence was generated by the GeneOptimizer recoding algorithm (Thermo Fisher Scientific) using the normal BRI1 sequence as a template (see the Supplementary Materials, data S2 for sequences). .. Protein fusions under the control of different promoters were generated by recombining pEN-L4-promoter-R1, pEN-L1-CDS-L2, and pEN-R2-CITRINE-L3 plasmids into a destination vector by Multisite Gateway LR reaction (Thermo Fisher Scientific). ..



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